Megamix-Plus FSC

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Reference 7802
Status RUO
Sample 500 µl beads
Quantity 50 tests
Detection Fluorescent
Volume 25 ml
Application New standardization tool to count microparticles
Form Megamix-Plus FSC is a mix of beads with the following diameters: 0.1 µm, 0.3 µm, 0.5 µm and 0.9 µm.
Megamix-Plus FSC is a mix of fluorescent beads of varied diameters selected to cover the theoretical MP size range (0.1 to 1 µm). Beads acquisition according to the following procedure allows setting the cytometer to study MP within a constant size region (0.3 - 1 µm-eq.) and getting reproducible MP counts. The intrinsic numerical ratio of 2:1 from the 0.3 µm to 0.5 µm beads helps fine tuning FSC threshold for those instruments which only provide coarse discontinuous adjustment (e.g. BC Gallios).

Finally, Megamix-Plus FSC also helps to transfer both the protocol and scatter settings from one cytometer to another and to check the stability over-time.

Date Document Name File size Download
2017/04/01 package insert Megamix-Plus FSC - 448.75 KB Download
Author Title Year of edition Review
Poncelet P. et alStandardized Counting of Circulating Platelet Microparticles Using Currently Available Flow Cytometers and Scatter-Based Triggering: Forward or Side Scatter?2016Cytometry Part A
Cointe S. et alStandardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop2016JTH
Sánchez-López VHigh correlation between 2 flow cytometry platforms in the microparticles analysis using a new calibrated beads strategy2015Transl Res.
Robert S et al. High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles 2012ATVB
Title Reference
Megamix-Plus SSC7803
MP-Count Beads7804

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