Megamix-Plus FSC

    default
    Reference
    7802
    Status
    RUO
    Sample
    500 µl beads
    Quantity
    50 tests
    Detection
    Fluorescent
    Volume
    25 ml
    Application
    Flow Cytometry Kits
    Form

    Megamix-Plus FSC is a mix of beads with the following diameters: 0.1 µm, 0.3 µm, 0.5 µm and 0.9 µm.

    Megamix-Plus FSC is a mix of fluorescent beads of varied diameters selected to cover the theoretical MP size range (0.1 to 1 µm). Beads acquisition according to the following procedure allows setting the cytometer to study MP within a constant size region (0.3 - 1 µm-eq.) and getting reproducible MP counts. The intrinsic numerical ratio of 2:1 from the 0.3 µm to 0.5 µm beads helps fine tuning FSC threshold for those instruments which only provide coarse discontinuous adjustment (e.g. BC Gallios).

    Finally, Megamix-Plus FSC also helps to transfer both the protocol and scatter settings from one cytometer to another and to check the stability over-time.

    Date Document Name Download
    01/04/2017 Package insert Megamix-Plus FSC EN (419.55 KB)
    FR (448.75 KB)
    Author Title Year of edition Review
    Robert S et al.
    High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles
    2012
    ATVB
    Poncelet P. et al
    Standardized Counting of Circulating Platelet Microparticles Using Currently Available Flow Cytometers and Scatter-Based Triggering: Forward or Side Scatter?
    2016
    Cytometry Part A
    Sánchez-López V
    High correlation between 2 flow cytometry platforms in the microparticles analysis using a new calibrated beads strategy
    2015
    Transl Res.
    Cointe S. et al
    Standardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop
    2016
    JTH