Principle
A microwell plate is coated with a specific mouse monoclonal antihuman VASP antibody that binds to VASP to be measured. Then, a peroxidase-coupled mouse anti-human VASP-P monoclonal antibody binds to phosphorylated serine 239 antigenic determinant of VASP. The bound enzyme peroxidase is then revealed by its activity on the TMB substrate over a predetermined time. After stopping the reaction, absorbance at 450 nm is directly related to the concentration of VASP-P initially contained in the sample.
BblioPresentation
| Reference |
CP013 & CP019 |
|---|---|
| Sample | 80 µL citrated whole blood |
| Quantity | 47 samples maximum |
| Detection |
ELISA |
| Reagents | PGE1, PGE1 + ADP, Lysis buffer, Coated plate, Washing solution, Dilution buffer, Anti-human VASP-P antibody –peroxidase, TMB, Stop solution. |
| Description | Enzyme immunoassay of VASP-P. |