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MEGAMIX

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Reference 7801
Status RUO
Sample 500 µl beads
Quantity 50 tests
Detection Fluorescent
Volume 25 ml
Application Standardization tool to count microparticles
Form Megamix is a mix of beads from chosen diameter : 0.5 µm, 0.9 µm and 3 µm.
Megamix is a mix of fluorescent beads of varied diameters to cover the MP (0.5 and 0.9 µm) and platelet size ranges (0.9 and 3 µm). This bead acquisition according to the following procedure allows the settings of the cytometer to study MP within a constant size region and to get reproducible MP counts. Megamix also allows to transfer the protocol and the settings from one cytometer to another and to guarantee the stability over time.
Date Document Name File size Download
2010/06/08 Poster Megamix CYTO2011 329 - 821.02 KB Download
2010/06/08 Poster Megamix CYTO2011 330 - 745.37 KB Download
2016/10/01 package insert Megamix - 128.51 KB Download
2010/06/08 Flyer Megamix - 27.56 KB Download
Author Title Year of edition Review
Poncelet P et alTips and tricks for flow cytometry-based analysis and counting of microparticles2015Transf Apher Sci
Robert S et al. High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles 2012ATVB
Amabile N. et al. Circulation immune complexes do not affect microparticle flow cytometry analysis in acute coronary syndrome 2012Blood
Stagnara J et al. Correlation between platelet-derived microparticle enumeration by flow cytometry and phospholipid-dependent procoagulant activity in microparticles: The centrifugation step matters! 2012Thromb Haemost.
Thomas GM et al.Cancer cell-derived microparticles bearing P-selectin glycoprotein ligand 1 accelerate thrombus formation in vivo2009J. Exp. Med.
Robert S et al.Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?2009J Thromb Haemost.
Mobarrez F et al.A multicolor flow cytometric assay for measurement of platelet-derived microparticles2009Thromb Res
Prokopi M et al.Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures2009Blood
Machado GBSExou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis2009Shock
Plotkowski MC et al.ExoU-induced procoagulant activity in Pseudomonas aeruginosa-infected airway cells2008Eur Respir J
Lawrie AS et al.The characterization and impact of microparticles on haemostasis within fresh-frozen plasma2008Vox Sanguinis
Title Reference
Megamix-Plus SSC7803
MP-Count Beads7804

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