Megamix-Plus FSC

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Reference
7802
Status
RUO
Sample
500 µl beads
Quantity
50 tests
Detection
Fluorescent
Volume
25 ml
Application
Flow Cytometry Kits
Form

Megamix-Plus FSC is a mix of beads with the following diameters: 0.1 µm, 0.3 µm, 0.5 µm and 0.9 µm.

Megamix-Plus FSC is a mix of fluorescent beads of varied diameters selected to cover the theoretical MP size range (0.1 to 1 µm). Beads acquisition according to the following procedure allows setting the cytometer to study MP within a constant size region (0.3 - 1 µm-eq.) and getting reproducible MP counts. The intrinsic numerical ratio of 2:1 from the 0.3 µm to 0.5 µm beads helps fine tuning FSC threshold for those instruments which only provide coarse discontinuous adjustment (e.g. BC Gallios).

Finally, Megamix-Plus FSC also helps to transfer both the protocol and scatter settings from one cytometer to another and to check the stability over-time.

Date Document Name Download
01/04/2017 Package insert Megamix-Plus FSC EN (419.55 KB)
FR (448.75 KB)
Author Title Year of edition Review
Robert S et al.
High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles
2012
ATVB
Poncelet P. et al
Standardized Counting of Circulating Platelet Microparticles Using Currently Available Flow Cytometers and Scatter-Based Triggering: Forward or Side Scatter?
2016
Cytometry Part A
Sánchez-López V
High correlation between 2 flow cytometry platforms in the microparticles analysis using a new calibrated beads strategy
2015
Transl Res.
Cointe S. et al
Standardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop
2016
JTH