CY-QUANT ELISA sCD146

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Reference
7501
Status
RUO
Sample
Serum, citrated or EDTA plasma.
Quantity
96 tests
Detection
Colorimetric
fiche specification
Colorimetric
gamme mesure
100 to 1,600 ng/ml
Application
Human ELISA Kits, ELISA Kits
Form

- 3 x 2 strips of Reagent 1 (Coated Strip) - 3 vials of Reagent 2 (Calibrator) - 1 vial of Reagent 3 (Washing solution) - 1 vial of Reagent 4 (Dilution buffer) - 1 vial of Reagent 5 (Anti CD146-peroxidase) - 1 vial of Reagent 6 (TMB) - 1 vial of Reagent 7 (Control plasma) - 1 vial of Reagent 8 (Stop solution) - 1 plate frame

A plastic support coated with specific mouse monoclonal anti human CD146 F(ab’)2 fragments (Reagent 1) binds to the sCD146 to be measured. Next the mouse monoclonal antibody anti CD146 coupled with peroxidase (Reagent 5) binds to a remaining free antigenic determinant of the CD146. The bound enzyme peroxidase is then revealed by its activity in a predetermined time on the TMB substrate (Reagent 6). After stopping the reaction (Reagent 8), the intensity of the signal is directly related to the concentration of sCD146 initially contained in the sample.

Date Document Name Download
01/04/2015 Package insert CY-QUANT ELISA sCD146 EN (80.30 KB)
FR (82.42 KB)
Author Title Year of edition Review
Bardin N et al.
Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration
2003
Thromb Haemost
Malyszko J. et al.
Adiponectin is related to CD146, a novel marker of endothelial cell activation/injury in chronic renal failure and peritoneally dialyzed patients
2003
JCEM
Malyszko J. et al.
Is there a link between CD146, a novel adhesion molecule and other markers of endothelial dysfunction in nephrotic syndrome and continuous ambulatory peritoneal dialysis?
2005
Thromb Res
Pasquier E et al.
The first assessment of soluble CD146 in women with unexplained pregnancy loss. A new insight?
2005
Thromb Haemost.
Figarella-Branger et al.
Platelet-endothelial cell adhesion molecule-1 and CD146: soluble levels and in situ expression of cellular adhesion molecules implicated in the cohesion of endothelial cells in idiopathic inflammatory myopathies
2006
J Rheumatol.
Wrobel T et al.
sVE-cadherin and sCD146 serum levels in patients with multiple myeloma
2006
Clin.Lab.Haem.
Bardin N et al.
Increased Expression of CD146, a New Marker of the Endothelial Junction in Active Inflammatory Bowel Diseas
2006
Inflamm Bowel Dis.
Malyszko J. et al.
Adipokines, Linking Adipocytes and Vascular Function in Hemodialyzed Patients, May Also Be Possibly Relatedto CD146, a Novel Adhesion Molecule
2007
Clin Appl Thromb Hemost.
Klakamp SL et al.
Application of Analytical Detection Concepts to Immunogenicity Testing
2007
Anal. Chem.
Reumaux D et al.
Restoration of soluble CD146 in patients with Crohn's disease treated with the TNF- antagonist infliximab (p NA)
2007
Inflamm Bowel Dis.
Bardin N et al.
CD146 and its Soluble Form Regulate Monocyte Transendothelial Migration
2009
Arterioscler Thromb Vasc Biol
Kaspi E et al.
Soluble CD146 and APS: a potential biomarker of obstetrical complications?
2012
Lupus
Dogansen S.C. et al
The relationship between early atherosclerosis and endothelial dysfunction in type 1 diabetic patients as evidenced by measurement of carotid
2013
Cardiovascular Diabetology
Ilie M et al
Clinical value of circulating endothelial cells and of soluble CD146 levels in patients undergoing surgery for non-small cell lung cancer
2014
Br J Cancer
Gayat et al
Soluble CD146, a new endothelial biomarker of acutely decompensated heart failure
2015
Int J Cardiol
Kubena P et al
Plasma Levels of Soluble CD146 Reflect the Severity of Pulmonary Congestion Better Than Brain Natriuretic Peptide in Acute Coronary Syndrome
2016
Ann Lab Med
Nollet M. et al
A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule
2017
Oncotarget
Von Moos S. et al.
Vascular endothelial growth factor D is a biomarker of fluid overload in haemodialysis patients
2020
Nephrol Dial Transplant.