Détail

CY-QUANT ELISA sCD146

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Reference 7501
Status RUO
Sample Serum, citrated or EDTA plasma.
Quantity 96 tests
Assay Range 100 to 1,600 ng/ml
Detection Colorimetric
Application Detection of soluble CD146 in plasma and serum
Form - 3 x 2 strips of Reagent 1 (Coated Strip) - 3 vials of Reagent 2 (Calibrator) - 1 vial of Reagent 3 (Washing solution) - 1 vial of Reagent 4 (Dilution buffer) - 1 vial of Reagent 5 (Anti CD146-peroxidase) - 1 vial of Reagent 6 (TMB) - 1 vial of Reagent 7 (Control plasma) - 1 vial of Reagent 8 (Stop solution) - 1 plate frame
A plastic support coated with specific mouse monoclonal anti human CD146 F(ab’)2 fragments (Reagent 1) binds to the sCD146 to be measured. Next the mouse monoclonal antibody anti CD146 coupled with peroxidase (Reagent 5) binds to a remaining free antigenic determinant of the CD146. The bound enzyme peroxidase is then revealed by its activity in a predetermined time on the TMB substrate (Reagent 6). After stopping the reaction (Reagent 8), the intensity of the signal is directly related to the concentration of sCD146 initially contained in the sample.
Date Document Name File size Download
2015/04/01 package insert CY-QUANT ELISA sCD146 - 82.42 KB Download
Author Title Year of edition Review
Nollet M. et alA novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule2017Oncotarget
Kubena P et alPlasma Levels of Soluble CD146 Reflect the Severity of Pulmonary Congestion Better Than Brain Natriuretic Peptide in Acute Coronary Syndrome2016Ann Lab Med
Gayat et alSoluble CD146, a new endothelial biomarker of acutely decompensated heart failure2015Int J Cardiol
Ilie M et alClinical value of circulating endothelial cells and of soluble CD146 levels in patients undergoing surgery for non-small cell lung cancer2014Br J Cancer
Dogansen S.C. et al The relationship between early atherosclerosis and endothelial dysfunction in type 1 diabetic patients as evidenced by measurement of carotid2013Cardiovascular Diabetology
Kaspi E et al. Soluble CD146 and APS: a potential biomarker of obstetrical complications? 2012Lupus
Bardin N et al. CD146 and its Soluble Form Regulate Monocyte Transendothelial Migration 2009Arterioscler Thromb Vasc Biol
Malyszko J. et al.Adipokines, Linking Adipocytes and Vascular Function in Hemodialyzed Patients, May Also Be Possibly Related to CD146, a Novel Adhesion Molecule2007Clin Appl Thromb Hemost.
Klakamp SL et al.Application of Analytical Detection Concepts to Immunogenicity Testing2007Anal. Chem.
Reumaux D et al.Restoration of soluble CD146 in patients with Crohn's disease treated with the TNF- antagonist infliximab (p NA)2007Inflamm Bowel Dis.
Figarella-Branger et al.Platelet-endothelial cell adhesion molecule-1 and CD146: soluble levels and in situ expression of cellular adhesion molecules implicated in the cohesion of endothelial cells in idiopathic inflammatory myopathies2006J Rheumatol.
Wrobel T et al.sVE-cadherin and sCD146 serum levels in patients with multiple myeloma2006Clin.Lab.Haem.
Bardin N et al.Increased Expression of CD146, a New Marker of the Endothelial Junction in Active Inflammatory Bowel Diseas2006Inflamm Bowel Dis.
Malyszko J. et al.Is there a link between CD146, a novel adhesion molecule and other markers of endothelial dysfunction in nephrotic syndrome and continuous ambulatory peritoneal dialysis?2005Thromb Res
Pasquier E et al.The first assessment of soluble CD146 in women with unexplained pregnancy loss. A new insight?2005Thromb Haemost.
Bardin N et al.Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration2003Thromb Haemost
Malyszko J. et al.Adiponectin is related to CD146, a novel marker of endothelial cell activation/injury in chronic renal failure and peritoneally dialyzed patients2003JCEM

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