Presentation
Reference
CP010
Sample
200 µL EDTA or heparin whole blood
Quantity
10 calibration curves
Detection
Fluorescent
Application
Flow Cytometry Kits
Form
Diluent, Calibration beads, Staining reagent, Neutralization solution, Lysing solution, Fixative solution
Principle
Whole blood is stained by indirect immunofluorescence and analyzed by quantitative flow cytometry. Counter-staining reagents can be added to the procedure to gate and analyze sub-populations of interest. The expression level of the tested IgG is determined using the calibration beads. HUMAN IgG Calibrator is also applicable on isolated cells.
Download
| Date | Document Name | Download |
|---|---|---|
| 01/01/2010 | Poster HUMAN IgG Calibrator CD33 | EN (1.39 MB) |
| 01/12/2011 | Poster Human IgG Calibrator ASH2011 | EN (407.85 KB) |
| 01/03/2016 | Package insert HUMAN IgG Calibrator | EN (67.99 KB) |
| HUMAN | Package | EN (67.99 KB) |
Bibliography
| Author | Title | Year of edition | Review |
|---|---|---|---|
|
A quantitative flow cytometric assay for determining binding characteristics of chimeric, humanized and human antibodies in whole blood: Proof of principle with rituximab and ofatumumab
|
2013
|
J Immunol Methods
|
|
|
How Validated Receptor Occupancy Flow Cytometry Assays Can Impact Decisions and Support Drug Development
|
2015
|
Cytometry Part B
|
|
|
Highly Potent, Anthracycline-based Antibody–Drug Conjugates Generated by Enzymatic, Site-specific Conjugation
|
2017
|
Mol Cancer Ther
|
|
|
CD20 and CD37 antibodies synergize to activate complement by Fc-mediated clustering
|
2019
|
Haematologica
|
Associated products
| Title | Reference |
|---|---|
RAT IgG Calibrator |
CP011
|