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CY-QUANT VASP/P2Y12

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Reference 7502
Status CE
Sample 160 µL citrated  whole blood
Quantity 1 à 23 tests/run
Detection ELISA
Application For the measurement of specific platelet ADP receptor antagonists (P2Y12)
Form Coated microtiter plate, PGE1, PGE1 + ADP, Lysis buffer,Washing solution, Dilution buffer, Anti-VASP-P peroxidase, TMB, Stop solution, tool to extract wells from their strips
CY-QUANT VASP/P2Y12 kit is dedicated to the measurement of specific platelet ADP receptor (P2Y12) antagonists. VASP (Vasodilator Stimulated Phosphoprotein) is an intracellular platelet protein which is unphosphorylated at basal state. Prostaglandin E1 (PGE1) induces phosphorylation of VASP, whereas the binding of adenosine diphosphate (ADP) to P2Y12 receptors leads to dephosphorylation of VASP. Under test conditions, in the concomitant addition of ADP and PGE1, the effect of ADP dominates and leads to VASP dephosphorylation, unless the P2Y12 receptor is efficiently blocked by antiplatelet drugs targeting this receptor (such as thienopyridines). Thus, the level of VASP phosphorylation in this condition reflects the level of P2Y12 receptor inhibition.
Date Document Name File size Download
2013/09/01 Flyer CY-QUANT VASP P2Y12 - 726.86 KB Download
2017/12/01 package insert CY-QUANT VASP P2Y12 - 135.36 KB Download
Author Title Year of edition Review
Deepak L. Bhatt et alAntibody-Based Ticagrelor Reversal Agent in Healthy Volunteers2019The New England Journal of Medicine
Holm M. et alThe MOVEMENT Trial2019Journal of the American Heart Association
Takehiko Nagao et alA noninferiority confirmatory trial of prasugrel versus clopidogrel in Japanese patients with non-cardioembolic stroke: rationale and study design for a randomized controlled trial – PRASTRO-I trial2018Expert Opinion on Pharmacotherapy
Ito Y. et alVasodilator-stimulated phosphoprotein (VASP) is not a major mediator of platelet aggregation, thrombogenesis, haemostasis, and antiplatelet effect of prasugrel in rats.2018International Journal of Scientific Reports
Singh D. & Silakari O.Facile alkylation of 4-nitrobenzotriazole and its platelet aggregation inhibitory activity2017Bioorg. Med. Chem.
Ding P. et al.Validation of a New ELISA-Based Vasodilator-Associated Stimulated Phosphoprotein Phosphorylation Assay to Assess Platelet Reactivity Index in a Chinese Population2017Clin Appl Thromb Hemost.
Deharo P. et alBenefit of Switching Dual Antiplatelet Therapy After Acute Coronary Syndrome According to On-Treatment Platelet Reactivity: The TOPIC-VASP Pre-Specified Analysis of the TOPIC Randomized Study2017JACC Cardiovasc Interv.
Bonello L. et alParameters of complete blood count do not predict on-treatment platelet reactivity in acute coronary syndrome patients.2017Thrombosis Research
Umemura K & Iwaki T. The Pharmacokinetics and Pharmacodynamics of Prasugrel and Clopidogrel in Healthy Japanese Volunteers2016Clin Pharmacol Drug Dev
Gaussem P et al.Inefficacy of Platelet Transfusion to Reverse Ticagrelor2015N Engl J Med
Söderlund et alIn vitro anti-platelet potency of ticagrelor in blood samples from infants and children2015Thromb Research
Bonello L et al Onset of optimal P2Y12-ADP receptor blockade after ticagrelor and prasugrel intake in Non-ST elevation acute coronary syndrome2015Thromb Haemost
Zeymer U. et alDouble-blind, randomized, prospective comparison of loading doses of 600 mg clopidogrel versus 60 mg prasugrel in patients with acute ST-segment elevation myocardial infarction scheduled for primary percutaneous intervention2015JACC: Cardiovascular Interventions
Abtan J et alIdentification of poor response to P2Y12 inhibitors in ACS patients with a new ELISA-based vasodilator-associated stimulated phosphoprotein2013Thromb Haemost
Hobl E.L et alComparison of a new ELISA-based with the flow cytometric assay for vasodilator-associated stimulated phosphoprotein (VASP) phosphorylation to assess P2Y12-inhibition after ticagrelor intake2013Cytometry
Jakubowski J.A. et alComparison of a new ELISA assay with the flow cytometric assay for platelet vasodilator-associated stimulated2012Thromb. Haemost
Barragan P. et alValidation of a novel ELISA-based VASP whole blood assay to measure P2Y12-ADP receptor activity2010Thromb. Haemost
Title Reference
PLT VASP/P2Y127014

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