Presentation
- Washing buffer and diluent, 10 fold concentrated - Negative isotypic control (mouse MAb, IgG) - Anti-CD38 MAb - Calibrated bead suspension. The beads are coated with increasing and accurately known quantities of mouse IgG - Staining reagent, polyclonal anti mouse IgG-FITC - Neutralization solution - CD8-PE
Principle
The immuno-labelling is achieved by adding to the sample the monoclonal antibody (MAb) specific to CD38. A staining reagent is added both to the sample and to the calibrator. Red cell lysis is performed after the addition of the CD8-PE counter-stain. The fluorescence intensity, proportional to the number of MAb CD38 bound to the lymphocyte surface, is converted into accessible sites using the calibrator and the negative isotypic control. The counter-staining allows to quantify CD38 antigen expression on CD8+ lymphocytes.
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