CY-QUANT ELISA sCD146

    default
    Reference
    7501
    Status
    RUO
    Sample
    Serum, citrated or EDTA plasma.
    Quantity
    96 tests
    Detection
    Colorimetric
    fiche specification
    Colorimetric
    gamme mesure
    100 to 1,600 ng/ml
    Application
    Human ELISA Kits, ELISA Kits
    Form

    - 3 x 2 strips of Reagent 1 (Coated Strip) - 3 vials of Reagent 2 (Calibrator) - 1 vial of Reagent 3 (Washing solution) - 1 vial of Reagent 4 (Dilution buffer) - 1 vial of Reagent 5 (Anti CD146-peroxidase) - 1 vial of Reagent 6 (TMB) - 1 vial of Reagent 7 (Control plasma) - 1 vial of Reagent 8 (Stop solution) - 1 plate frame

    A plastic support coated with specific mouse monoclonal anti human CD146 F(ab’)2 fragments (Reagent 1) binds to the sCD146 to be measured. Next the mouse monoclonal antibody anti CD146 coupled with peroxidase (Reagent 5) binds to a remaining free antigenic determinant of the CD146. The bound enzyme peroxidase is then revealed by its activity in a predetermined time on the TMB substrate (Reagent 6). After stopping the reaction (Reagent 8), the intensity of the signal is directly related to the concentration of sCD146 initially contained in the sample.

    Date Document Name Download
    01/04/2015 Package insert CY-QUANT ELISA sCD146 EN (80.30 KB)
    FR (82.42 KB)
    Author Title Year of edition Review
    Bardin N et al.
    Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration
    2003
    Thromb Haemost
    Malyszko J. et al.
    Adiponectin is related to CD146, a novel marker of endothelial cell activation/injury in chronic renal failure and peritoneally dialyzed patients
    2003
    JCEM
    Malyszko J. et al.
    Is there a link between CD146, a novel adhesion molecule and other markers of endothelial dysfunction in nephrotic syndrome and continuous ambulatory peritoneal dialysis?
    2005
    Thromb Res
    Pasquier E et al.
    The first assessment of soluble CD146 in women with unexplained pregnancy loss. A new insight?
    2005
    Thromb Haemost.
    Figarella-Branger et al.
    Platelet-endothelial cell adhesion molecule-1 and CD146: soluble levels and in situ expression of cellular adhesion molecules implicated in the cohesion of endothelial cells in idiopathic inflammatory myopathies
    2006
    J Rheumatol.
    Wrobel T et al.
    sVE-cadherin and sCD146 serum levels in patients with multiple myeloma
    2006
    Clin.Lab.Haem.
    Bardin N et al.
    Increased Expression of CD146, a New Marker of the Endothelial Junction in Active Inflammatory Bowel Diseas
    2006
    Inflamm Bowel Dis.
    Malyszko J. et al.
    Adipokines, Linking Adipocytes and Vascular Function in Hemodialyzed Patients, May Also Be Possibly Relatedto CD146, a Novel Adhesion Molecule
    2007
    Clin Appl Thromb Hemost.
    Klakamp SL et al.
    Application of Analytical Detection Concepts to Immunogenicity Testing
    2007
    Anal. Chem.
    Reumaux D et al.
    Restoration of soluble CD146 in patients with Crohn's disease treated with the TNF- antagonist infliximab (p NA)
    2007
    Inflamm Bowel Dis.
    Bardin N et al.
    CD146 and its Soluble Form Regulate Monocyte Transendothelial Migration
    2009
    Arterioscler Thromb Vasc Biol
    Kaspi E et al.
    Soluble CD146 and APS: a potential biomarker of obstetrical complications?
    2012
    Lupus
    Dogansen S.C. et al
    The relationship between early atherosclerosis and endothelial dysfunction in type 1 diabetic patients as evidenced by measurement of carotid
    2013
    Cardiovascular Diabetology
    Ilie M et al
    Clinical value of circulating endothelial cells and of soluble CD146 levels in patients undergoing surgery for non-small cell lung cancer
    2014
    Br J Cancer
    Gayat et al
    Soluble CD146, a new endothelial biomarker of acutely decompensated heart failure
    2015
    Int J Cardiol
    Kubena P et al
    Plasma Levels of Soluble CD146 Reflect the Severity of Pulmonary Congestion Better Than Brain Natriuretic Peptide in Acute Coronary Syndrome
    2016
    Ann Lab Med
    Nollet M. et al
    A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule
    2017
    Oncotarget
    Von Moos S. et al.
    Vascular endothelial growth factor D is a biomarker of fluid overload in haemodialysis patients
    2020
    Nephrol Dial Transplant.